Although Tao's published results highlight the power of electrochemical imaging microscopy to uncover explosive residues, he notes that the method can be usefully applied to a full assortment of detection applications. His group is currently using electrochemical imaging microscopy to monitor the activities of living cells, as well as to detect protein biomarkers—early warning beacons that can alert clinicians to pre-symptomatic signs of disease. This could offer improved speed and a lower cost for biomarker discovery when compared with current microarray approaches. Other potential uses include detection of heavy metal ions in drinking water.

The technique dispenses with the traditional microelectrode used for chemical sensing. "The key idea here," Tao explains, "is to convert an optical signal into local electrochemical current." This is accomplished thanks to a phenomenon known as surface plasmon resonance.

In an electrode—a metal conductor through which electric current is passed—electrons move freely and oscillate in a wavelike fashion called a plasmon. Shining light on the surface plasmon causes the electrons to absorb energy and enter an excited state. Tao notes that the plasmon is exquisitely sensitive to any changes occurring near the electrode's surface. If, for example, an electrochemical reaction involving oxidation or reduction takes place (where electrons are lost or gained, respectively), the plasmon registers this change as a reflection of light (electrochemical current can be inferred from the changes in optical signals detected). The technique allows for the resolution of trace chemicals down to a small fraction of a micron in diameter.

The TNT experiments were carried out by first depositing a fingerprint on the surface of an electrode. The raised ridges of the fingerprint formed a delicate layer of protein that blocked the flow of electrochemical current, whereas the grooves allowed current to flow, providing the contrast to reveal the fingerprint in vivid relief when an electrical potential was applied.

Next, the applied potential was lowered to correspond to the specific reduction potential of TNT, at which point spots of the explosive particles appeared, providing both visual and chemical confirmation. Remarkably, the technique could successfully detect the grains of TNT, even if they were mixed with other species of particles, including traces of dust, airborn particulate matter or wax.